Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections.
نویسندگان
چکیده
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.
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عنوان ژورنال:
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
دوره 17 49 شماره
صفحات -
تاریخ انتشار 2012